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Promega 5 × reaction buffer a v307a-c
5 × Reaction Buffer A V307a C, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reaction+buffer+c/kinase+assay/us11787801-380-70-71
Average 90 stars, based on 1 article reviews
5 × reaction buffer a v307a-c - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Incubation:

Article Title: Genetically modified Bt maize lines containing cry3Bb1, cry1A105 or cry1Ab2 do not affect the structure and functioning of root-associated endophyte communities
Article Snippet: Transgenic maize expressing Bacillus thuringiensis (Bt) insecticidal crystal (Cry) proteins may contribute to a decreased need for chemical pesticides, providing benefits for human health and the environment.. However, there is public concern regarding the environmental safety of transgenic crops, which relates also to potential adverse effects on plant-microbe interactions by affecting the plant-associated, endophytic microflora.. Thus, we compared the endophytic bacterial communities in plants of the three transgenic Bt maize lines MON89034 (cry3Bb1), MON88017 (cry1A.105 and cry2Ab2) and the stacked event MON88017xMON89034 (cry1A.105 and cry2Ab2, cry3Bb1), with those of the respective near-isogenic line, and with those in plants of three additional, conventional maize lines.

Amplification:

Article Title: Sediment Bacterial Communities Reflect the History of a Sea Basin
Article Snippet: The triplicate products were pooled and purified, using an EZNA Cycle Pure kit (Omega Bio-Tek Inc., Norcross, GA, USA) for both T-RFLP and cloning. .. Two hundred ng of the amplification products were digested with 5 U of the restriction enzyme HaeIII (several products were also cut separately with HhaI, MspI and RsaI, Promega Corp., Madison, WI, USA) in reaction buffer C (Promega) for 4 h at 37°C. .. One μl of the restriction digestion product was mixed with 0.2 μl of MapMarker® 1000 (Bioventures Inc., Murfreesboro, TN, USA) internal size standard and 20 μl of HiDi formamide (Applied Biosystems, Foster City, CA, USA) and denatured at 95°C for 5 min.

Article Title: Phosphorus Chemistry and Bacterial Community Composition Interact in Brackish Sediments Receiving Agricultural Discharges
Article Snippet: If non-specific amplification products were formed, amplicons were purified from 1% agarose gel using MinEluteTM Gel extraction kit (Qiagen, Hilden Germany). .. In all, 200 ng of purified and labelled amplification products were digested separately with 5 U of the HaeIII, HhaI, MspI and RsaI restriction enzymes (Promega Corp., Madison, WI, USA) in reaction buffer C for 2 h at 37°C. ..

Purification:

Article Title: Phosphorus Chemistry and Bacterial Community Composition Interact in Brackish Sediments Receiving Agricultural Discharges
Article Snippet: If non-specific amplification products were formed, amplicons were purified from 1% agarose gel using MinEluteTM Gel extraction kit (Qiagen, Hilden Germany). .. In all, 200 ng of purified and labelled amplification products were digested separately with 5 U of the HaeIII, HhaI, MspI and RsaI restriction enzymes (Promega Corp., Madison, WI, USA) in reaction buffer C for 2 h at 37°C. ..

other:

Article Title: Genetic diversity in the blackberry rust pathogen, Phragmidium violaceum, in Europe and Australasia as revealed by analysis of SAMPL.
Article Snippet: Indigenous to Europe, the blackberry rust fungus Phragmidium violaceum was introduced to Australia and subsequently appeared in New Zealand, with the most recent authorised introductions to Australia specifically for the biological control of European blackberry.. Markers for ‘selective amplification of microsatellite polymorphic loci’ (SAMPL) were developed for studying the population genetics of P. violaceum.. Modification of one of the two SAMPL primers with a HaeIII adapter (H) revealed significantly greater levels of genetic variation than primers used to generate AFLPs, the latter revealing little or no variation among 25 Australasian and 19 European isolates of P. violaceum.



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